pAGD289
(Plasmid
#228113)
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PurposepET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21 (+)
- Backbone size w/o insert (bp) 5342
- Total vector size (bp) 6695
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKva1 retron, ncRNA with 90 bp rpoB donor
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SpeciesKlebsiella variicola
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Insert Size (bp)1323
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGD289 was a gift from Seth Shipman (Addgene plasmid # 228113 ; http://n2t.net/addgene:228113 ; RRID:Addgene_228113) -
For your References section:
An experimental census of retrons for DNA production and genome editing. Khan AG, Rojas-Montero M, Gonzalez-Delgado A, Lopez SC, Fang RF, Crawford KD, Shipman SL. Nat Biotechnol. 2024 Sep 17. doi: 10.1038/s41587-024-02384-z. 10.1038/s41587-024-02384-z PubMed 39289529
