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Addgene

p16 nlsGPS2
(Plasmid #228093)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228093 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p16-Kan vector
  • Backbone manufacturer
    Jan Lohmann (PMID: 24576426)
  • Vector type
    Plant Expression
  • Selectable markers
    Neomycin (select with G418), URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nlsGPS2
  • Alt name
    nuclear localized GIBBERELLIN PERCEPTION SENSOR 2
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    2840
  • Promoter p16
  • Tags / Fusion Proteins
    • 6xHis-T7 XpressTag
    • cMyc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Improved version of the GA biosensor GPS1 (Addgene plasmid # 101084)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p16 nlsGPS2 was a gift from Alexander Jones (Addgene plasmid # 228093 ; http://n2t.net/addgene:228093 ; RRID:Addgene_228093)
  • For your References section:

    GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth. Griffiths J, Rizza A, Tang B, Frommer WB, Jones AM. Plant Cell. 2024 Jul 23:koae198. doi: 10.1093/plcell/koae198. 10.1093/plcell/koae198 PubMed 39039020