p16 nlsGPS2
(Plasmid
#228093)
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PurposeExpression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228093 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep16-Kan vector
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Backbone manufacturerJan Lohmann (PMID: 24576426)
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418), URA3
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenlsGPS2
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Alt namenuclear localized GIBBERELLIN PERCEPTION SENSOR 2
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SpeciesA. thaliana (mustard weed), Synthetic
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Insert Size (bp)2840
- Promoter p16
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Tags
/ Fusion Proteins
- 6xHis-T7 XpressTag
- cMyc (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Improved version of the GA biosensor GPS1 (Addgene plasmid # 101084)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p16 nlsGPS2 was a gift from Alexander Jones (Addgene plasmid # 228093 ; http://n2t.net/addgene:228093 ; RRID:Addgene_228093) -
For your References section:
GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth. Griffiths J, Rizza A, Tang B, Frommer WB, Jones AM. Plant Cell. 2024 Jul 23:koae198. doi: 10.1093/plcell/koae198. 10.1093/plcell/koae198 PubMed 39039020