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Addgene

mCit-APPAPAPPAP-gs
(Plasmid #228091)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCit, APPAPAPPAP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a 3bp indel between His tag and FRB in the Addgene NGS result compared to the given reference.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCit-APPAPAPPAP-gs was a gift from Roman Jerala (Addgene plasmid # 228091 ; http://n2t.net/addgene:228091 ; RRID:Addgene_228091)

  • For your References section:

    Programmable de novo designed coiled coil-mediated phase separation in mammalian cells. Ramsak M, Ramirez DA, Hough LE, Shirts MR, Vidmar S, Elersic Filipic K, Anderluh G, Jerala R. Nat Commun. 2023 Dec 2;14(1):7973. doi: 10.1038/s41467-023-43742-w. 10.1038/s41467-023-43742-w PubMed 38042897
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