mCit-APPAPAPPAP-gs
(Plasmid
#228091)
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PurposeMammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 228091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCit, APPAPAPPAP
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a 3bp indel between His tag and FRB in the Addgene NGS result compared to the given reference.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCit-APPAPAPPAP-gs was a gift from Roman Jerala (Addgene plasmid # 228091 ; http://n2t.net/addgene:228091 ; RRID:Addgene_228091) -
For your References section:
Programmable de novo designed coiled coil-mediated phase separation in mammalian cells. Ramsak M, Ramirez DA, Hough LE, Shirts MR, Vidmar S, Elersic Filipic K, Anderluh G, Jerala R. Nat Commun. 2023 Dec 2;14(1):7973. doi: 10.1038/s41467-023-43742-w. 10.1038/s41467-023-43742-w PubMed 38042897
