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NST3_pOpOn2.1
(Plasmid #228066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOpOn2.1
  • Backbone manufacturer
    Dr. Markéta Šámalová
  • Backbone size w/o insert (bp) 18688
  • Total vector size (bp) 19765
  • Vector type
    Plant Expression ; Dexamethasone inducible vector
  • Selectable markers
    Kanamycin for plants

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use 50 µg/ml Spectinomycin for bacterial selection and 25 µg/ml of Kanamycin for plant selection
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3
  • Alt name
    SECONDARY WALL-ASSOCIATED NAC DOMAIN 1, SND1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1077
  • Entrez Gene
    NAC012 (a.k.a. AT1G32770, ANAC012, F6N18.15, F6N18_15, NAC SECONDARY WALL THICKENING PROMOTING 3, NAC domain containing protein 12, NST3, SECONDARY WALL-ASSOCIATED NAC DOMAIN 1, SND1)
  • Promoter CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GCAAGACCCTTCCTCTATATAAGG
  • 3′ sequencing primer GCAGGACTCTAGTGGATCCC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Markéta Šámalová originally generated this vector and deposited it into NASC stock centre. We purchased it from NASC (2109482) and cloned full length NST3 into it.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A H162R mutation was found in GUS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NST3_pOpOn2.1 was a gift from Charles Anderson (Addgene plasmid # 228066 ; http://n2t.net/addgene:228066 ; RRID:Addgene_228066)

  • For your References section:

    NST3 induces ectopic transdifferentiation, forming secondary walls with diverse patterns and composition in Arabidopsis thaliana. Tamadaddi C, Choi J, Ghasemi M, Kim SH, Gomez ED, Gomez EW, Anderson CT. Ann Bot. 2024 Aug 30:mcae153. doi: 10.1093/aob/mcae153. 10.1093/aob/mcae153 PubMed 39212164
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