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Addgene

pGL4.26-Igf2Promoter
(Plasmid #228060)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228060 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL4.26
  • Backbone manufacturer
    Promega
  • Vector type
    Luciferase
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Igf2 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    910
  • GenBank ID
  • Entrez Gene
    Igf2 (a.k.a. Igf-2, Igf-II, M6pr, Mpr, Peg2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.26-Igf2Promoter was a gift from Kenneth Chen (Addgene plasmid # 228060 ; http://n2t.net/addgene:228060 ; RRID:Addgene_228060)
  • For your References section:

    An imbalance between proliferation and differentiation underlies the development of microRNA-defective pineoblastoma. Fraire CR, Desai K, Obalapuram UA, Mendyka LK, Rajaram V, Sebastian T, Wang Y, Onel K, Lee J, Chen KS. bioRxiv [Preprint]. 2024 Apr 30:2024.04.23.590638. doi: 10.1101/2024.04.23.590638. 10.1101/2024.04.23.590638 PubMed 38712047