pGL4.26-Igf2Promoter
(Plasmid
#228060)
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Purpose0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4.26
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Backbone manufacturerPromega
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Vector typeLuciferase
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIgf2 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)910
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GenBank ID
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Entrez GeneIgf2 (a.k.a. Igf-2, Igf-II, M6pr, Mpr, Peg2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.26-Igf2Promoter was a gift from Kenneth Chen (Addgene plasmid # 228060 ; http://n2t.net/addgene:228060 ; RRID:Addgene_228060) -
For your References section:
An imbalance between proliferation and differentiation underlies the development of microRNA-defective pineoblastoma. Fraire CR, Desai K, Obalapuram UA, Mendyka LK, Rajaram V, Sebastian T, Wang Y, Onel K, Lee J, Chen KS. bioRxiv [Preprint]. 2024 Apr 30:2024.04.23.590638. doi: 10.1101/2024.04.23.590638. 10.1101/2024.04.23.590638 PubMed 38712047