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pLXC2B-PLAG1-3xFLAG
(Plasmid #228057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX303-cyan-2a-blast
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLAG1-3xFLAG
  • Alt name
    PLAG1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1569
  • Entrez Gene
    PLAG1 (a.k.a. PSA, SGPA, SRS4, ZNF912)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3XFLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLXC2B-PLAG1-3xFLAG was a gift from James Amatruda & Kenneth Chen (Addgene plasmid # 228057 ; http://n2t.net/addgene:228057 ; RRID:Addgene_228057)

  • For your References section:

    Mutations in microRNA processing genes in Wilms tumors derepress the IGF2 regulator PLAG1. Chen KS, Stroup EK, Budhipramono A, Rakheja D, Nichols-Vinueza D, Xu L, Stuart SH, Shukla AA, Fraire C, Mendell JT, Amatruda JF. Genes Dev. 2018 Aug 1;32(15-16):996-1007. doi: 10.1101/gad.313783.118. Epub 2018 Jul 19. 10.1101/gad.313783.118 PubMed 30026293
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