pUCmini-RELC-PGK-tdTomato
(Plasmid
#228056)
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PurposeHomologous donor template for RELC knockout expressing tdTomato
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUCmini
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato
- Promoter PGK
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer GTGTTCCGCATTCTGCAAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCmini-RELC-PGK-tdTomato was a gift from Yohei Sato (Addgene plasmid # 228056 ; http://n2t.net/addgene:228056 ; RRID:Addgene_228056) -
For your References section:
CRISPR/Cas9-mediated RELA and RELC knockout in human regulatory T cells abrogates FOXP3 expression and suppressive function. Sato Yohei, Hanawa Yamato, Tsubota Akihito. Clinical Immunology Communications, Volume 6, 2024 10.1016/j.clicom.2024.10.002