pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter)
(Plasmid
#228043)
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PurposeDONR vector for inflammasome speck reporter, designed from PMC9623368.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
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Vector typeGateway DONR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCASP1[CARD]-mSG2
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made byJenster et al. (2023); Inflammasome speck reporter was designed from PMC9623368
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 CASP1[CARD]-mSG2 (inflammasome reporter) was a gift from Wade Harper (Addgene plasmid # 228043 ; http://n2t.net/addgene:228043 ; RRID:Addgene_228043)