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Addgene

mRFP-LAP2β
(Plasmid #228029)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228029 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    n/a
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LAP2b
  • Alt name
    Lamina-associated polypeptide 2, isoform beta
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Tmpo (a.k.a. LAP2)
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Lentiviral construct for mRFP-LAP2β expression was cloned by inserting the DNA sequence of mRFP-LAP2β (Addgene plasmid 21047, from D. Gerlich) into a lentiviral expression vector carrying a hygromycin resistance marker

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mRFP-LAP2β was a gift from David Pellman (Addgene plasmid # 228029 ; http://n2t.net/addgene:228029 ; RRID:Addgene_228029)
  • For your References section:

    A tubule-sheet continuum model for the mechanism of nuclear envelope assembly. Zhao G, Liu S, Arun S, Renda F, Khodjakov A, Pellman D. Dev Cell. 2023 May 22;58(10):847-865.e10. doi: 10.1016/j.devcel.2023.04.003. Epub 2023 Apr 24. 10.1016/j.devcel.2023.04.003 PubMed 37098350