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Addgene

*MtbCM-HiBiT-His
(Plasmid #228028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6132
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Rv1885c Chorismate Mutase
  • Alt name
    *MtbCM – Secreted mycobacterium tuberculosis chorismate mutase
  • Alt name
    *MtCM
  • Alt name
    Rv1885c chorismate mutase [ Mycobacterium tuberculosis H37Rv ]
  • Species
    Mycobacterium tuberculosis H37Rv
  • Insert Size (bp)
    714
  • GenBank ID
  • Entrez Gene
    Rv1885c (a.k.a. Rv1885c, *MtCM)
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • 10xHis-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Hind3 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We had Genscript prepare this plasmid by substituting the HiBIT sequence for the AviTag (Bio) in the MtbCM-Bio-His-pET1a+ plasmid (Addgene plasmid # 186027)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    *MtbCM-HiBiT-His was a gift from James Inglese (Addgene plasmid # 228028 ; http://n2t.net/addgene:228028 ; RRID:Addgene_228028)

  • For your References section:

    Active- and Allosteric-Site Cyclic Peptide Inhibitors of Secreted M. tuberculosis Chorismate Mutase. van Neer RHP, Dranchak PK, Aitha M, Liu L, Carlson EK, Jacobsen IE, Battaile K, Fang Y, Tao D, Rai G, Padia J, Lovell S, Suga H, Inglese J. ACS Infect Dis. 2025 Mar 14;11(3):703-714. doi: 10.1021/acsinfecdis.4c00798. Epub 2025 Feb 4. 10.1021/acsinfecdis.4c00798 PubMed 39903128
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