pLPC MYC hTel2
(Plasmid
#22802)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLPC-N MYC
- Backbone size w/o insert (bp) 5600
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTelomere Maintenance 2
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Alt nameTel2
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Alt nameTelo2
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Alt nameCLK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2511
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GenBank IDNM_016111
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Entrez GeneTELO2 (a.k.a. CLK2, TEL2, YHFS)
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Tag
/ Fusion Protein
- MYC (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer TAGGCGTGTACGGTGGGA
- 3′ sequencing primer CTTGCCAAACCTACAGGT (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLPC MYC hTel2 was a gift from Titia de Lange (Addgene plasmid # 22802 ; http://n2t.net/addgene:22802 ; RRID:Addgene_22802) -
For your References section:
Tel2 regulates the stability of PI3K-related protein kinases. Takai H, Wang RC, Takai KK, Yang H, de Lange T. Cell. 2007 Dec 28. 131(7):1248-59. 10.1016/j.cell.2007.10.052 PubMed 18160036