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Addgene

pLPC MYC hTel2
(Plasmid #22802)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22802 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLPC-N MYC
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Telomere Maintenance 2
  • Alt name
    Tel2
  • Alt name
    Telo2
  • Alt name
    CLK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2511
  • GenBank ID
    NM_016111
  • Entrez Gene
    TELO2 (a.k.a. CLK2, TEL2, YHFS)
  • Tag / Fusion Protein
    • MYC (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC MYC hTel2 was a gift from Titia de Lange (Addgene plasmid # 22802 ; http://n2t.net/addgene:22802 ; RRID:Addgene_22802)
  • For your References section:

    Tel2 regulates the stability of PI3K-related protein kinases. Takai H, Wang RC, Takai KK, Yang H, de Lange T. Cell. 2007 Dec 28. 131(7):1248-59. 10.1016/j.cell.2007.10.052 PubMed 18160036