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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22801 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXI
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Backbone manufacturerClontech
- Backbone size (bp) 6809
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10F', 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEmpty vector
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not I (not destroyed)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer QCXIforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIZ (w298-1) was a gift from Eric Campeau (Addgene plasmid # 22801 ; http://n2t.net/addgene:22801 ; RRID:Addgene_22801)