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Addgene

pRS416-ADH1p-BirA-FRB-HA3
(Plasmid #227984)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227984 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA-FRB
  • Species
    Other
  • Promoter ADH1
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS416-ADH1p-BirA-FRB-HA3 was a gift from Joseph Reese (Addgene plasmid # 227984 ; http://n2t.net/addgene:227984 ; RRID:Addgene_227984)

  • For your References section:

    Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex. Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC. G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221. 10.1093/g3journal/jkae221 PubMed 39271111
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