pFA6a-mTID-HA3-HIS3MX6
(Plasmid
#227983)
-
PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 227983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepRS416
-
Vector typeYeast Expression
-
Selectable markersHIS3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameminiTurboID
-
SpeciesOther
-
Tag
/ Fusion Protein
- HA tag (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFA6a-mTID-HA3-HIS3MX6 was a gift from Joseph Reese (Addgene plasmid # 227983 ; http://n2t.net/addgene:227983 ; RRID:Addgene_227983) -
For your References section:
Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex. Pfannenstein J, Tyryshkin M, Gulden ME, Doud EH, Mosley AL, Reese JC. G3 (Bethesda). 2024 Sep 13:jkae221. doi: 10.1093/g3journal/jkae221. 10.1093/g3journal/jkae221 PubMed 39271111