Ai3
(Plasmid #22797)

• Depositing Lab: Hongkui Zeng
• Publication: Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Backbone

• Vector backbone: Rosa26 Targeting Vector
• Backbone size w/o insert (bp): 9862
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Best grown in bacterial strain that minimizes recombination (i.e., STBL2).
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CAG-Floxed EYFP
• Species: n/a
• Insert Size (bp): 6319

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Backbone - Liqun Luo; EYFP - Invitrogen
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry