pTW357d5
(Plasmid
#227931)
-
PurposeHigh-stringency accessory plasmid for selection
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 227931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonen/a
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert namegIII (recoded)
-
SpeciesEscherichia phage fd
Cloning Information for Gene/Insert 1
- Cloning method Other
Gene/Insert 2
-
Gene/Insert nameT7c
-
SpeciesE. coli
-
MutationR632S, Q649S
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTW357d5 was a gift from Tina Wang (Addgene plasmid # 227931 ; http://n2t.net/addgene:227931 ; RRID:Addgene_227931)