pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT
(Plasmid
#227907)
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PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 227907 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTwist Amp High Copy
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Backbone manufacturerTwist Biosciences
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT was a gift from Manuel Leonetti (Addgene plasmid # 227907 ; http://n2t.net/addgene:227907 ; RRID:Addgene_227907) -
For your References section:
Global organelle profiling reveals subcellular localization and remodeling at proteome scale. Hein MY, Peng D, Todorova V, McCarthy F, Kim K, Liu C, Savy L, Januel C, Baltazar-Nunez R, Sekhar M, Vaid S, Bax S, Vangipuram M, Burgess J, Njoya L, Wang E, Ivanov IE, Byrum JR, Pradeep S, Gonzalez CG, Aniseia Y, Creery JS, McMorrow AH, Sunshine S, Yeung-Levy S, DeFelice BC, Mehta SB, Itzhak DN, Elias JE, Leonetti MD. Cell. 2024 Dec 26:S0092-8674(24)01344-8. doi: 10.1016/j.cell.2024.11.028. 10.1016/j.cell.2024.11.028 PubMed 39742809