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pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT
(Plasmid #227900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist Amp High Copy
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT was a gift from Manuel Leonetti (Addgene plasmid # 227900 ; http://n2t.net/addgene:227900 ; RRID:Addgene_227900)

  • For your References section:

    Global organelle profiling reveals subcellular localization and remodeling at proteome scale. Hein MY, Peng D, Todorova V, McCarthy F, Kim K, Liu C, Savy L, Januel C, Baltazar-Nunez R, Sekhar M, Vaid S, Bax S, Vangipuram M, Burgess J, Njoya L, Wang E, Ivanov IE, Byrum JR, Pradeep S, Gonzalez CG, Aniseia Y, Creery JS, McMorrow AH, Sunshine S, Yeung-Levy S, DeFelice BC, Mehta SB, Itzhak DN, Elias JE, Leonetti MD. Cell. 2024 Dec 26:S0092-8674(24)01344-8. doi: 10.1016/j.cell.2024.11.028. 10.1016/j.cell.2024.11.028 PubMed 39742809
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