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Addgene

p-miR-Report + WT miR-181 BS in NLK 3UTR
(Plasmid #22790)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22790 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p-miR-Report
  • Backbone manufacturer
    Ambion
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Wild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region
  • Alt name
    WT miR-181 BS in NLK 3UTR
  • Alt name
    WT NLK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    38
  • Entrez Gene
    NLK

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer AGGCGATTAAGTTGGGTA
  • 3′ sequencing primer ATTGCAACGATTTAGGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-miR-Report + WT miR-181 BS in NLK 3UTR was a gift from Xin Wang (Addgene plasmid # 22790 ; http://n2t.net/addgene:22790 ; RRID:Addgene_22790)

  • For your References section:

    Identification of microRNA-181 by genome-wide screening as a critical player in EpCAM-positive hepatic cancer stem cells. Ji J, Yamashita T, Budhu A, Forgues M, Jia HL, Li C, Deng C, Wauthier E, Reid LM, Ye QH, Qin LX, Yang W, Wang HY, Tang ZY, Croce CM, Wang XW. Hepatology. 2009 Aug . 50(2):472-80. 10.1002/hep.22989 PubMed 19585654
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