pCPCbLCVB.002
(Plasmid
#22787)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript SK+ II (original backbone)
- Backbone size w/o insert (bp) 6854
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Vector typeRNAi ; Arabidopsis VIGS vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha or any strain for high quality plasmid DNA
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameB DNA
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Alt nameCbLCV VIGS vector
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Alt nameCaLCuV VIGS vector
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Alt nameCaLCuV B-DNA VIGS
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MutationN/A
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GenBank IDAY279344
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byErnest Heibert and James Strandberg, University of Florida, Gainesville, FL provided the wild type viral vector.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCPCbLCVB.002 was a gift from Niki Robertson (Addgene plasmid # 22787 ; http://n2t.net/addgene:22787 ; RRID:Addgene_22787) -
For your References section:
Geminivirus-based vectors for gene silencing in Arabidopsis. Turnage MA, Muangsan N, Peele CG, Robertson D. Plant J. 2002 Apr . 30(1):107-14. 10.1046/j.1365-313X.2002.01261.x PubMed 11967097