pCPCbLCV.007
(Plasmid #22785)

• Depositing Lab: Niki Robertson
• Publication: Turnage et al Plant J. 2002 Apr . 30(1):107-14.

Backbone

• Vector backbone: pBluescript SK+ II (original backbone)
• Backbone size w/o insert (bp): 5352
• Vector type: RNAi ; Arabidopsis VIGS vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha or any strain for high quality plasmid DNA
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: A DNA (with MCS)+
• Alt name: CbLCV VIGS vector
• Alt name: CaLCuV VIGS vector
• Alt name: CaLCuV A-DNA VIGS
• Insert Size (bp): 800
• GenBank ID: AY279345

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ernest Heibert and James Strandberg, University of Florida, Gainesville, FL provided the wild type viral vector.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The coat protein gene was replaced with a polylinker containing Acc65I. Up to 800 bp can be inserted for VIGS. Released episome is 1.9 kb without insert.

Must be bombarded with appropriate B DNA for systemic infection