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pHAGE-EF1a-HSF1-GFP
(Plasmid #227820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HSF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1587
  • Entrez Gene
    HSF1 (a.k.a. HSTF1)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • GFP (C terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AfeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human HSF1-GFP lentivirus vector expressed from UBc promoter was a gift from Maria Vera Ugalde (McGill University, Canada). Here, we replace the promoter to EF1a.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.10.05.616786 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-EF1a-HSF1-GFP was a gift from Jeffrey Gerst (Addgene plasmid # 227820 ; http://n2t.net/addgene:227820 ; RRID:Addgene_227820)

  • For your References section:

    Conservation of mRNA operon formation in the control of the heat shock response in mammalian cells. Pataki E, Gerst JE. bioRxiv 2024.10.05.616786 10.1101/2024.10.05.616786
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