pNX24-RFP-MS2SL
(Plasmid #227819)

• Purpose: Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene
• Depositing Lab: Jeffrey Gerst
• Publication: Haimovich et al bioRxiv 2024.11.06.622258

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: 3xHA-RFP-24xMS2SL
• Species: Synthetic
• Insert Size (bp): 2128
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: -

Gene/Insert 2

• Gene/Insert name: Neo resistance
• Species: Synthetic
• Insert Size (bp): 792
• Promoter: SV40

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • Haimovich_DL_1_pNX24-MS2L_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.

Please note: This plasmid is prone to recombination and removal of some of the MS2 repeats. Please select multiple colonies for screening for the full length plasmid before performing experiments.