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Addgene

pCyPet-C1
(Plasmid #22779)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCyPet-C1-Control
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CyPet
  • Species
    A. victoria
  • Insert Size (bp)
    720
  • Mutation
    Control for intermolecular Rac1 biosensor FRET. Similar to pEGFP-C1, which was recloned to have CyPet.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site Bgl II (not destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCyPet-C1 was a gift from Klaus Hahn (Addgene plasmid # 22779 ; http://n2t.net/addgene:22779 ; RRID:Addgene_22779)

  • For your References section:

    Coordination of Rho GTPase activities during cell protrusion. Machacek M, Hodgson L, Welch C, Elliott H, Pertz O, Nalbant P, Abell A, Johnson GL, Hahn KM, Danuser G. Nature. 2009 Sep 3. 461(7260):99-103. 10.1038/nature08242 PubMed 19693013
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