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Addgene

p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2
(Plasmid #227775)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227775 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Tol2
  • Total vector size (bp) 15690
  • Vector type
    zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    BFP-MCS
  • Insert Size (bp)
    756
  • Promoter Olactb

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    mCherry-t2A-CreERT2
  • Insert Size (bp)
    3016
  • Promoter hsp70l

Cloning Information for Gene/Insert 2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2 was a gift from Bushra Raj (Addgene plasmid # 227775 ; http://n2t.net/addgene:227775 ; RRID:Addgene_227775)
  • For your References section:

    Barcoding Notch signaling in the developing brain. Siniscalco AM, Perera RP, Greenslade JE, Veeravenkatasubramanian H, Masters A, Doll HM, Raj B. Development. 2024 Nov 22:dev.203102. doi: 10.1242/dev.203102. 10.1242/dev.203102 PubMed 39575683