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Addgene

p155ME-loxP-dsR-loxP-Cas9-t2A-GFP
(Plasmid #227774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Middle entry Cas9-t2A-GFP
  • Total vector size (bp) 8941
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    loxP-dsR-loxP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1253

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p155ME-loxP-dsR-loxP-Cas9-t2A-GFP was a gift from Bushra Raj (Addgene plasmid # 227774 ; http://n2t.net/addgene:227774 ; RRID:Addgene_227774)

  • For your References section:

    Barcoding Notch signaling in the developing brain. Siniscalco AM, Perera RP, Greenslade JE, Veeravenkatasubramanian H, Masters A, Doll HM, Raj B. Development. 2024 Nov 22:dev.203102. doi: 10.1242/dev.203102. 10.1242/dev.203102 PubMed 39575683
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