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p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA
(Plasmid #227773)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tol2
  • Total vector size (bp) 12211
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9-t2A-GFP
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    5276
  • Promoter tp1

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    4xU6:sgRNA
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2115
  • Promoter U6

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer ACATTAAATGAAATGCATcagt
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA was a gift from Bushra Raj (Addgene plasmid # 227773 ; http://n2t.net/addgene:227773 ; RRID:Addgene_227773)

  • For your References section:

    Barcoding Notch signaling in the developing brain. Siniscalco AM, Perera RP, Greenslade JE, Veeravenkatasubramanian H, Masters A, Doll HM, Raj B. Development. 2024 Nov 22:dev.203102. doi: 10.1242/dev.203102. 10.1242/dev.203102 PubMed 39575683
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