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Addgene

pVG329
(Plasmid #227771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS40H
  • Vector type
    Yeast Expression
  • Selectable markers
    hphMX (hygromycine resistance in budding yeast)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10
  • Growth instructions
    Can also be grown in DH5alpha.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Genomic sequences flanking YOR1 in budding yeast
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    N/A
  • Entrez Gene
    YOR1 (a.k.a. YGR281W, YRS1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For deletion of YOR1 in buddign yeast genome, the plasmid should be linearized with EcoRV. For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVG329 was a gift from Sahand Jamal Rahi (Addgene plasmid # 227771 ; http://n2t.net/addgene:227771 ; RRID:Addgene_227771)

  • For your References section:

    Light-directed evolution of dynamic, multi-state, and computational protein functionalities. Gligorovski V, Labagnara M, Rahi SJ. bioRxiv 2024.02.28.582517 10.1101/2024.02.28.582517
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