MSCV CreERT2 puro
(Plasmid #22776)

• Depositing Lab: Tyler Jacks
• Publication: Kumar et al Genes Dev. 2009 Nov 10. ():.

Backbone

• Vector backbone: pMSCV puro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CreERT2
• Alt name: Cre-ER-T2
• Alt name: Cre-ERT2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MSCV

Resource Information

• Articles Citing this Plasmid:
  • 28 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cre-ERT2 contains Cre fused to the ligand-binding domain of a mutated estrogen receptor, which recognizes tamoxifen or its derivative 4-hydroxytamoxifen(4-OHT). This construct permits tamoxifen-dependent Cre activity.

Please note that Addgene's sequencing results identified a K183E mutation in the Cre protein sequence. This mutation was not intentional, but was present in the original plasmid and according to the depositing laboratory the plasmid functions as described in the associated publication.