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Addgene

pVG147
(Plasmid #227753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EZL105
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3 ; Cg (budding yeast)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10
  • Growth instructions
    Can also be grown in DH5alpha.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EL222 codon-optimized for budding yeast
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Gly94Cys mutant
  • Promoter PGK1pr

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For a single-copy integration in budding yeast, the plasmid should be cut with PmeI (double-cutter). Please visit https://doi.org/10.1101/2024.02.28.582517 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVG147 was a gift from Sahand Jamal Rahi (Addgene plasmid # 227753 ; http://n2t.net/addgene:227753 ; RRID:Addgene_227753)

  • For your References section:

    Light-directed evolution of dynamic, multi-state, and computational protein functionalities. Gligorovski V, Labagnara M, Rahi SJ. bioRxiv 2024.02.28.582517 10.1101/2024.02.28.582517
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