Fret-Stop-Fret TOPO
(Plasmid #22774)

• Depositing Lab: Tyler Jacks
• Publication: Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

Backbone

• Vector backbone: pCR-Blunt II-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: However, plasmid yield and stability is improved when grown at 30 degrees.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: fret-STOP-fret
• Alt name: FRT-STOP-FRT
• Alt name: FSF
• Insert Size (bp): 5600

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid containing FRT-STOP-FRT cassette for use in expressing genes only after Flp-mediated recombination.

It's like the LSL cassette ( http://www.addgene.org/11584 but this one is a FSF cassette