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LAG3-DEM (L3#1)
(Plasmid #227739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPIX
  • Backbone size w/o insert (bp) 5798
  • Total vector size (bp) 7511
  • Vector type
    Mammalian Expression, Bacterial Expression, TALEN
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Designer epigenome modifier targeting LAG3-promoter (Homo sapiens)
  • Alt name
    LAG3-DEM (L3#1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4314
  • Promoter CMV
  • Tag / Fusion Protein
    • DNMT3A, DNMT3L, KRAB (N terminal on backbone)

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer gccgtggaagccgtgc
  • 3′ sequencing primer gctgggtcgggtctgc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LAG3-DEM (L3#1) was a gift from Claudio Mussolino (Addgene plasmid # 227739 ; http://n2t.net/addgene:227739 ; RRID:Addgene_227739)

  • For your References section:

    Protocol for Efficient Generation of Chimeric Antigen Receptor T Cells with Multiplexed Gene Silencing by Epigenome Editing. Azcona MSR, Mussolino C. Methods Mol Biol. 2024;2842:209-223. doi: 10.1007/978-1-0716-4051-7_11. 10.1007/978-1-0716-4051-7_11 PubMed 39012598
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