Skip to main content
Addgene

pDest-pol2-Smarcd1-Myc
(Plasmid #227716)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDest
  • Backbone manufacturer
    Dom Esposito
  • Backbone size w/o insert (bp) 8600
  • Total vector size (bp) 9700
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smarcd1
  • Alt name
    Baf60
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • GenBank ID
    NM_031842.2
  • Entrez Gene
    Smarcd1 (a.k.a. Baf60a, D15Kz1)
  • Promoter pol2
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CACCATGGCGGCCCGGGCGGGTTT
  • 3′ sequencing primer CTATGTGTTTCGGATTCCCAGGGCTTGCTCTAACTCTTGCCGCCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.24.577061 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest-pol2-Smarcd1-Myc was a gift from Kent Hunter (Addgene plasmid # 227716 ; http://n2t.net/addgene:227716 ; RRID:Addgene_227716)

  • For your References section:

    SMARCD1 is an essential expression-restricted metastasis modifier. Ross C, Gong L-Y, Jenkins LM, Ha N-H, Majocha M, Hunter K. bioRxiv 2024.01.24.577061 10.1101/2024.01.24.577061
Related items: