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Addgene

pUT_mNF-Cas13d-Triplex
(Plasmid #227709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUt-mNF-Cas13d
  • Backbone manufacturer
    Balazsi Lab (Addgene # 224789)
  • Total vector size (bp) 11901
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-P2A-RfxCas13d-Triplex
  • Species
    Synthetic
  • Insert Size (bp)
    3944
  • Promoter CMV-d2i

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.11.593702 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUT_mNF-Cas13d-Triplex was a gift from Gabor Balazsi (Addgene plasmid # 227709 ; http://n2t.net/addgene:227709 ; RRID:Addgene_227709)

  • For your References section:

    Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting. Wan Y, Helenek C, Coraci D, Balazsi G. ACS Synth Biol. 2024 Oct 18;13(10):3212-3230. doi: 10.1021/acssynbio.4c00271. Epub 2024 Oct 8. 10.1021/acssynbio.4c00271 PubMed 39377757
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