pCDNA3.1-nv5-ER beta
(Plasmid #22770)

• Depositing Lab: Donald McDonnell
• Publication: Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

Backbone

• Vector backbone: pCDNA3.1/nv5-DEST
• Backbone size w/o insert (bp): 7136
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: estrogen receptor beta
• Species: H. sapiens (human)
• Insert Size (bp): 2555
• GenBank ID: NM_001437
• Entrez Gene: ESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
• Tag / Fusion Protein:
  • v5 (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry