pICH41331::pU6cm-pegR::EURb7
(Plasmid
#227695)
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PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepICH41331
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing lab recommends using 10beta strain for expression.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAltered epegRNA backbone with cloning sites for gRNA and RTT.
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SpeciesSynthetic
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Insert Size (bp)2287
- Promoter U6 composite
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer GTCAAGGCGGCGAAGTATT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICH41331::pU6cm-pegR::EURb7 was a gift from Jae-Yean Kim (Addgene plasmid # 227695 ; http://n2t.net/addgene:227695 ; RRID:Addgene_227695) -
For your References section:
Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Vu TV, Nguyen NT, Kim J, Song YJ, Nguyen TH, Kim JY. Nat Plants. 2024 Sep 6. doi: 10.1038/s41477-024-01786-w. 10.1038/s41477-024-01786-w PubMed 39242983
