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BCL2alpha-MJK2243
(Plasmid #227690)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMJK2243
  • Backbone size w/o insert (bp) 10753
  • Total vector size (bp) 11448
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human BCL-2alpha
  • Alt name
    BCL2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    720
  • GenBank ID
    NM_000633.3 NP_000624.2
  • Entrez Gene
    BCL2 (a.k.a. Bcl-2, PPP1R50)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CTCAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer CCACATAGCGTAAAAGGAGCAACATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BCL2alpha-MJK2243 was a gift from Curt Civin (Addgene plasmid # 227690 ; http://n2t.net/addgene:227690 ; RRID:Addgene_227690)

  • For your References section:

    The polypharmacy combination of the BCL-2 inhibitor venetoclax (VEN) and the FLT3 inhibitor gilteritinib (GIL) is more active in acute myeloid leukemia cells than novel polypharmacologic BCL-2/FLT3 VEN-GIL hybrid single-molecule inhibitors. Goodis CC, Eberly C, Chan AM, Kim M, Lowe BD, Civin CI, Fletcher S. Eur J Med Chem. 2024 Dec 22;285:117190. doi: 10.1016/j.ejmech.2024.117190. 10.1016/j.ejmech.2024.117190 PubMed 39813774
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