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pAc5.1B-EGFP-DmAGO1
(Plasmid #22768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Elisa Izaurralde
  • Backbone size w/o insert (bp) 6063
  • Vector type
    Drosophila expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Dh5 alpha and XL1-blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Argonaut-1, variant A
  • Alt name
    AGO1
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2955
  • GenBank ID
    NM_166020
  • Entrez Gene
    AGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago-1, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Ac5 forward primer site
  • 3′ sequencing primer BGH reverse priming site
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmAGO1 was a gift from Elisa Izaurralde (Addgene plasmid # 22768 ; http://n2t.net/addgene:22768 ; RRID:Addgene_22768)

  • For your References section:

    GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Eulalio A, Huntzinger E, Izaurralde E. Nat Struct Mol Biol. 2008 Apr . 15(4):346-53. 10.1038/nsmb.1405 PubMed 18345015
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