pAc5.1B-EGFP-DmAGO1
(Plasmid
#22768)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAc5.1B-EGFP
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Backbone manufacturerElisa Izaurralde
- Backbone size w/o insert (bp) 6063
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5 alpha and XL1-blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameArgonaut-1, variant A
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Alt nameAGO1
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2955
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GenBank IDNM_166020
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Entrez GeneAGO1 (a.k.a. Dmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel\CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Ac5 forward primer site
- 3′ sequencing primer BGH reverse priming site (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1B-EGFP-DmAGO1 was a gift from Elisa Izaurralde (Addgene plasmid # 22768 ; http://n2t.net/addgene:22768 ; RRID:Addgene_22768) -
For your References section:
GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Eulalio A, Huntzinger E, Izaurralde E. Nat Struct Mol Biol. 2008 Apr . 15(4):346-53. 10.1038/nsmb.1405 PubMed 18345015