PEmaxNG-_2star_IVT
(Plasmid
#227678)
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Purposeplasmid for SpCas9-NG-PEmax** mRNA IVT
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNG-PEmax_2_star
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SpeciesSynthetic
- Promoter T7
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Tag
/ Fusion Protein
- no
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer polyA-seq (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PEmaxNG-_2star_IVT was a gift from Scot Wolfe (Addgene plasmid # 227678 ; http://n2t.net/addgene:227678 ; RRID:Addgene_227678)
Map uploaded by the depositor.
