HEK4-53bp-tag-insertion-pegRNA
(Plasmid
#227673)
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PurposePegRNA for 53 bp insertion
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 227673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepbluescript
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSpCas9 sgRNA scaffold
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Alt nameHEK4 sgRNA
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gRNA/shRNA sequenceUnknown
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SpeciesH. sapiens (human)
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Entrez GeneEPHA3 (a.k.a. EK4, ETK, ETK1, HEK, HEK4, TYRO4)
- Promoter U6
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Rev
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HEK4-53bp-tag-insertion-pegRNA was a gift from Scot Wolfe (Addgene plasmid # 227673 ; http://n2t.net/addgene:227673 ; RRID:Addgene_227673)
Map uploaded by the depositor.
