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HEK4-53bp-tag-insertion-pegRNA
(Plasmid #227673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pbluescript
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpCas9 sgRNA scaffold
  • Alt name
    HEK4 sgRNA
  • gRNA/shRNA sequence
    Unknown
  • Species
    H. sapiens (human)
  • Entrez Gene
    EPHA3 (a.k.a. EK4, ETK, ETK1, HEK, HEK4, TYRO4)
  • Promoter U6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HEK4-53bp-tag-insertion-pegRNA was a gift from Scot Wolfe (Addgene plasmid # 227673 ; http://n2t.net/addgene:227673 ; RRID:Addgene_227673)

  • For your References section:

    Increasing intracellular dNTP levels improves prime editing efficiency. Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA. Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x. 10.1038/s41587-024-02405-x PubMed 39322763
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