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Addgene

pFNC-6
(Plasmid #227669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA6213
  • Backbone size w/o insert (bp) 3740
  • Total vector size (bp) 6904
  • Modifications to backbone
    Introduced sacB in the gadget site; introduced BxbI phage integrase in the MCS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    BxbI phage integrase
  • Species
    BxbI phage
  • Insert Size (bp)
    1503
  • Promoter PEM7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sacB
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    1422

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFNC-6 was a gift from Pablo Ivan Nikel (Addgene plasmid # 227669 ; http://n2t.net/addgene:227669 ; RRID:Addgene_227669)

  • For your References section:

    CIFR (Clone-Integrate-Flip-out-Repeat): A toolset for iterative genome and pathway engineering of Gram-negative bacteria. Federici F, Luppino F, Aguilar-Vilar C, Mazaraki ME, Petersen LB, Ahonen L, Nikel PI. Metab Eng. 2025 Jan 6;88:180-195. doi: 10.1016/j.ymben.2025.01.001. 10.1016/j.ymben.2025.01.001 PubMed 39778677
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