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Addgene

pAM200
(Plasmid #227659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS305
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpn5
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPN5 (a.k.a. YDL147W, NAS5)
  • Tag / Fusion Protein
    • 3X FLAG-HRV3C- (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM200 was a gift from Andreas Martin (Addgene plasmid # 227659 ; http://n2t.net/addgene:227659 ; RRID:Addgene_227659)

  • For your References section:

    Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation. Greene ER, Goodall EA, de la Pena AH, Matyskiela ME, Lander GC, Martin A. Elife. 2019 Nov 28;8:e49806. doi: 10.7554/eLife.49806. 10.7554/eLife.49806 PubMed 31778111
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