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Addgene

pAM54
(Plasmid #227653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Rpn11
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    amino acids 2-239, L132A
  • Entrez Gene
    RPN11 (a.k.a. YFR004W, MPR1)
  • Promoter T7
  • Tag / Fusion Protein
    • H6-PreScission (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Rpn8
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    1-179
  • Entrez Gene
    RPN8 (a.k.a. YOR261C)
  • Tag / Fusion Protein
    • PreScission-Strep (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM54 was a gift from Andreas Martin (Addgene plasmid # 227653 ; http://n2t.net/addgene:227653 ; RRID:Addgene_227653)

  • For your References section:

    Structure of the Rpn11-Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation. Worden EJ, Padovani C, Martin A. Nat Struct Mol Biol. 2014 Mar;21(3):220-7. doi: 10.1038/nsmb.2771. Epub 2014 Jan 23. 10.1038/nsmb.2771 PubMed 24463465
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