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Addgene

pBEST-PrOR1OR2-MGapt-UTR1-deGFP
(Plasmid #227648)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227648 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBEST
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    KL740
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MG aptamer (MGapt) and deGFP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.22.533877 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEST-PrOR1OR2-MGapt-UTR1-deGFP was a gift from Richard Murray (Addgene plasmid # 227648 ; http://n2t.net/addgene:227648 ; RRID:Addgene_227648)

  • For your References section:

    Metabolic perturbations to an E. coli-based cell-free system reveal a trade-off between transcription and translation. Kapasiawala M, Murray RM. bioRxiv 2023.03.22.533877 10.1101/2023.03.22.533877
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