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Addgene

PTet-F30-Pepper aptamer-UTR1-mTurq2
(Plasmid #227645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DVK
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MG1655-Z1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.22.533877 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PTet-F30-Pepper aptamer-UTR1-mTurq2 was a gift from Richard Murray (Addgene plasmid # 227645 ; http://n2t.net/addgene:227645 ; RRID:Addgene_227645)

  • For your References section:

    Metabolic perturbations to an E. coli-based cell-free system reveal a trade-off between transcription and translation. Kapasiawala M, Murray RM. bioRxiv 2023.03.22.533877 10.1101/2023.03.22.533877
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