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Addgene

pYFJ84
(Plasmid #227644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET16
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5711
  • Total vector size (bp) 7317
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10F'
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Acyl-Acyl carrier protein synthetase
  • Alt name
    AasS
  • Species
    Vibrio harveyi
  • Insert Size (bp)
    1664
  • Promoter T7
  • Tag / Fusion Protein
    • 10xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer Novagen T7 promoter primer TAATACGACTCACTA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    My former student Dr Yanfang Jiang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The enzyme is used to make substrates for fatty acid, polyketide and nonribosomal polypetide enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFJ84 was a gift from John Cronan (Addgene plasmid # 227644 ; http://n2t.net/addgene:227644 ; RRID:Addgene_227644)

  • For your References section:

    The soluble acyl-acyl carrier protein synthetase of Vibrio harveyi B392 is a member of the medium chain acyl-CoA synthetase family. Jiang Y, Chan CH, Cronan JE. Biochemistry. 2006 Aug 22;45(33):10008-19. doi: 10.1021/bi060842w. 10.1021/bi060842w PubMed 16906759
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