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Addgene

pAM71
(Plasmid #227621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET DUET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Rpn11
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    amino acids 24-160-GGSGSG-192-221
  • Entrez Gene
    RPN11 (a.k.a. YFR004W, MPR1)
  • Promoter T7
  • Tag / Fusion Protein
    • His-Prescission (N terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    rpn8
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    1-179
  • Entrez Gene
    RPN8 (a.k.a. YOR261C)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM71 was a gift from Andreas Martin (Addgene plasmid # 227621 ; http://n2t.net/addgene:227621 ; RRID:Addgene_227621)

  • For your References section:

    An AAA Motor-Driven Mechanical Switch in Rpn11 Controls Deubiquitination at the 26S Proteasome. Worden EJ, Dong KC, Martin A. Mol Cell. 2017 Sep 7;67(5):799-811.e8. doi: 10.1016/j.molcel.2017.07.023. Epub 2017 Aug 24. 10.1016/j.molcel.2017.07.023 PubMed 28844860
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