pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
(Plasmid #227610)

• Purpose: Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
• Depositing Lab: Luke Chao
• Publication: Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10.1016/j.jbc.2024.107740.

Backbone

• Vector backbone: pPICZ A
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SLC25A46
• Species: H. sapiens (human)
• Entrez Gene: SLC25A46 (a.k.a. HMSN6B, PCH1E)
• Promoter: AOX1
• Tag / Fusion Protein:
  • 10xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: MFN2
• Species: H. sapiens (human)
• Mutation: contains aa 2-400, 706-757 [TM and HB2 deleted]
• Entrez Gene: MFN2 (a.k.a. CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF, MSL)
• Promoter: AOX1
• Tag / Fusion Protein:
  • Twin-StrepTag (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry