pAc5.1A-Dm-miR-13a
(Plasmid #22761)

• Depositing Lab: Elisa Izaurralde
• Publication: Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Backbone

• Vector backbone: pAc5.1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5348
• Vector type: Drosophila expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Dh5 alpha and XL1-blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: micro RNA 13a
• Alt name: miR-13a
• Species: D. melanogaster (fly); primary transcript
• Insert Size (bp): 382
• GenBank ID: NR_002027
• Entrez Gene: mir-13a (a.k.a. Dmel_CR32931, CR32931, Dmel\CR32931, MiR-13a, miR-13, miR-13a, miR13a)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: Ac5 forward primer site
• 3′ sequencing primer: BGH reverse priming site

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry