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pPICZ A-SLC-His+Strep-Opa1 (SB254)
(Plasmid #227603)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227603 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZ A
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SLC25A46
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC25A46 (a.k.a. HMSN6B, PCH1E)
  • Promoter AOX1
  • Tag / Fusion Protein
    • 10xHis (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Opa1
  • Species
    H. sapiens (human)
  • Mutation
    contains aa 253-960 only
  • Promoter AOX1
  • Tag / Fusion Protein
    • Twin-StrepTag (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPICZ A-SLC-His+Strep-Opa1 (SB254) was a gift from Luke Chao (Addgene plasmid # 227603 ; http://n2t.net/addgene:227603 ; RRID:Addgene_227603)

  • For your References section:

    Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Opa1 and Mfn2. Boopathy S, Luce BE, Lugo CM, Hakim P, McDonald J, Kim HL, Ponce J, Ueberheide BM, Chao LH. J Biol Chem. 2024 Aug 31:107740. doi: 10.1016/j.jbc.2024.107740. 10.1016/j.jbc.2024.107740 PubMed 39222684
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